Research Articles
CRISPR-Cas9 Knockout Cell Line Generation: A 2025 Guide from Fundamentals to Clinical Applications
This article provides a comprehensive guide for researchers and drug development professionals on generating CRISPR-Cas9 knockout cell lines.
CRISPR-Cas9 Delivery Methods: A Comprehensive Guide for Therapeutic Development
This article provides a detailed overview of the current landscape of CRISPR-Cas9 delivery systems, tailored for researchers, scientists, and drug development professionals.
A Comprehensive Guide to gRNA Design Tools: Boosting CRISPR Efficiency and Specificity
This article provides a systematic guide to guide RNA (gRNA) design tools for researchers and drug development professionals utilizing CRISPR technology.
CRISPR Genome Editing: A Foundational Guide for Research and Therapeutic Development
This article provides a comprehensive introduction to CRISPR genome editing for researchers and drug development professionals.
CRISPR-Cas9 Double-Strand Break Formation: Molecular Mechanisms, Applications, and Clinical Translation
This article provides a comprehensive analysis of the CRISPR-Cas9 mechanism for double-strand break (DSB) formation, tailored for researchers, scientists, and drug development professionals.
From Bacterial Defense to Genetic Scalpel: The Complete History and Clinical Transformation of CRISPR
This article traces the remarkable journey of CRISPR-Cas systems from their initial discovery as a bacterial immune mechanism to their current status as a transformative technology in biomedicine.
HDR vs. NHEJ: A Strategic Guide to DNA Repair Pathways for CRISPR Genome Editing and Therapy
This article provides a comprehensive analysis of the two primary DNA double-strand break repair pathways, Non-Homologous End Joining (NHEJ) and Homology-Directed Repair (HDR), with a focus on their application in...
PAM Sequences in CRISPR: The Essential Gatekeeper of Genome Targeting and Therapeutic Development
This article provides a comprehensive exploration of the Protospacer Adjacent Motif (PAM) and its pivotal role in CRISPR-Cas systems.
The Complete Guide to gRNA Design: From Foundational Principles to Clinical Application in CRISPR Genome Editing
This article provides a comprehensive resource for researchers, scientists, and drug development professionals on the design and function of guide RNA (gRNA) in CRISPR systems.
CRISPR-Cas9: A Step-by-Step Guide to Mechanism, Applications, and Optimization for Research and Therapeutics
This article provides a comprehensive guide to CRISPR-Cas9 gene editing, detailing its foundational mechanism derived from bacterial immunity and its step-by-step workflow from design to analysis.