Research Articles
Evaluating Bioinformatics Pipeworks for Taxonomic Classification: A 2025 Guide for Biomedical Researchers
Accurate taxonomic classification is foundational for microbiome research, clinical diagnostics, and drug development.
Breaking the Species Barrier: Advanced Strategies for High-Resolution Microbiome Analysis in Biomedical Research
Achieving species-level resolution in microbiome data is a critical frontier for unlocking the full potential of microbiome research in drug discovery and therapeutic development.
Primer Selection for 16S rRNA Gene Sequencing: A Foundational Guide to Optimizing Microbiome Data Quality and Accuracy
Accurate profiling of microbial communities via 16S rRNA gene sequencing is fundamentally dependent on primer selection, a choice that introduces significant bias and influences all downstream conclusions.
Unlocking Microbial Dark Matter: How Next-Generation Sequencing Reveals the Hidden World of Unculturable Microbes
Next-generation sequencing (NGS) has revolutionized our ability to study the vast majority of microorganisms that cannot be cultivated in the laboratory.
Advanced Strategies to Boost HDR Efficiency in CRISPR Genome Editing
Homology-Directed Repair (HDR) is essential for precise CRISPR-based knock-ins but remains a major bottleneck due to its low efficiency compared to error-prone repair pathways.
Strategic Control of Cas9 Expression: A Comprehensive Guide to Minimizing Off-Target Effects in Genome Editing
This article provides a detailed examination of strategies to control Cas9 expression for the purpose of minimizing off-target effects in CRISPR-Cas9 genome editing.
Optimized CRISPR RNP Transfection Protocol: A Step-by-Step Guide for High-Efficiency Genome Editing
This comprehensive guide details optimized ribonucleoprotein (RNP) transfection protocols for CRISPR genome editing, tailored for researchers and drug development professionals.
Ex Vivo vs. In Vivo CRISPR Delivery: A Strategic Guide for Therapeutic Development
This article provides a comprehensive analysis of ex vivo and in vivo CRISPR-Cas9 delivery strategies for therapeutic applications.
Chromatin Accessibility and gRNA Efficiency: A Foundational Guide for CRISPR Researchers
This article provides a comprehensive analysis of how local chromatin accessibility fundamentally influences the efficiency of CRISPR-Cas9 gene editing.
Troubleshooting Low CRISPR Knockout Efficiency: A 2025 Guide for Researchers
This guide provides a comprehensive, up-to-date framework for researchers and drug development professionals to diagnose, troubleshoot, and validate low CRISPR-Cas9 knockout efficiency.